|Source:||The herbs of Desmodium styracifolium (Osh.) Merr.|
|Biological Activity or Inhibitors:||1. Schaftoside has antioxidant activity.
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.7715 mL||8.8576 mL||17.7151 mL||35.4302 mL||44.2878 mL|
|5 mM||0.3543 mL||1.7715 mL||3.543 mL||7.086 mL||8.8576 mL|
|10 mM||0.1772 mL||0.8858 mL||1.7715 mL||3.543 mL||4.4288 mL|
|50 mM||0.0354 mL||0.1772 mL||0.3543 mL||0.7086 mL||0.8858 mL|
|100 mM||0.0177 mL||0.0886 mL||0.1772 mL||0.3543 mL||0.4429 mL|
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Aug 1;932:66-73.
|Simultaneous determination of four flavonoids and one phenolic acid in rat plasma by LC-MS/MS and its application to a pharmacokinetic study after oral administration of the Herba Desmodii Styracifolii extract[Pubmed: 23831698]|
|A sensitive and selective liquid chromatography and tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of four flavonoids (Schaftoside, isovitexin, luteolin, and apigenin) and one phenolic acid (ferulic acid) in rat plasma using sulfamethoxazole as the internal standard (IS). The separation was performed using a Diamonsil C18 column, which was eluted with methanol (A) and 0.1‰ acetic acid (B). The gradient condition was as follows: 0-5min, 40-60% A; 5-6min, 60-95% A; and 6-10min, maintained at 95% A. The analytes were detected using a hybrid quadrupole linear ion trap mass spectrometer that was equipped with an electrospray ionization source in the negative ion and multiple-reaction monitoring modes. A full validation of the method was performed. The linearity of the analytical response was good, with correlation coefficients greater than 0.9925 for all of the compounds within the concentration range. The lower limits of quantitation (LLOQ) of Schaftoside, isovitexin, luteolin, apigenin, and ferulic acid in rat plasma were 1.66, 0.84, 3.69, 1.70, and 3.91ng/mL, respectively. The intra-day and inter-day precisions of the investigated components exhibited an RSD within 13.20%, and the accuracy (RE%) ranged from -8.47% to 10.90%. The results indicated that the developed method is sufficiently reliable for the pharmacokinetic study of Schaftoside, isovitexin, apigenin, luteolin, and ferulic acid in rats following oral administration of the Herba Desmodii Styracifolii extract.|
Zuckerindustrie Sugar Industry, 2015, 140(10):632-639.
|An HPLC-DPPH method for antioxidant activity from sugarcane molasses.[Reference: WebLink]|
|Sugarcane molasses is potentially rich in health-promoting phenolic compounds. Present study was designed to optimize experimental conditions for ultrasonic-assisted extraction of antioxidant compounds from sugarcane molasses using response surface methodology. An HPLCDPPH method for simultaneous determination of antioxidant activity was also developed. Ethanol concentration 80-84% (v/v), temperature 58-59 degrees C, and 47-50 min time duration were the most favorable conditions. In these conditions, the optimal experimental results were total phenolic content 18 mg gallic acid equivalents/g, with DPPH free radical scavenging ability of 92%, which was same as the predicted values by RSM model. Catechin, vanillic acid, isorhamnetin-3-O-glucoside, eugenol, Schaftoside, diosmetin-7-O-beta-D-glucopyranoside, ferulic acid, and caffeoylquinic acid were identified using HPLC-MS/MS. Among the antioxidant compounds, Schaftoside was the most abundant antioxidant (92.081 mu g/g dry substance), while ferulic acid exhibited the highest antioxidant activity. These results suggest that HPLC-DPPH method is more specific, accurate and less time consuming and can sever as a quality control tool.|
Food Chem. 2016 Oct 1;208:89-96.
|Flour from Prosopis alba cotyledons: A natural source of nutrient and bioactive phytochemicals.[Pubmed: 27132827 ]|
|The Prosopis alba seed is a waste material in the process to produce pod flour. To suggest a potential use of these seeds it is necessary to determine the nutritional, phytochemical and functional quality of cotyledon flour from Prosopis alba. This flour showed high level of proteins (62%), low content of total carbohydrate and fat. Free polyphenol (1150±20mg GAE/100g flour) and carotenoids (10.55±0.05mg β-CE/100g flour) compounds were the dominant compounds. The main identified constituents in the polyphenolic extracts were C- glycosyl flavones, including Schaftoside, isoSchaftoside, vicenin II, vitexin and isovitexin. The extract enriched in polyphenolic compounds exhibited ABTS(+) reducing capacity and scavenging activity of H2O2; and was able to inhibit phospholipase, lipoxygenase and cyclooxygenase, three pro-inflammatory enzymes. According to our results, the P. alba cotyledon flour could be considered as a new alternative in the formulation of functional foods or food supplements.|