| Structure Identification: |
| Chinese Journal of Pharmaceutical Analysis, 2015,35(2):210-217. | | Determination of triterpenoids in Rhizoma Alismatis extracts.[Reference: WebLink] | To establish ultraviolet-visible(UV-Vis)spectrophotometry and HPLC method for determination of triterpenoids in Rhizoma Alismatis extracts.
METHODS AND RESULTS:
With 5% vanillin-acetic acid and perchloric acid as the developer, the colorimetric method was applied.Total triterpenoids in extracts was determined at 555 nm wavelength by UV-Vis spectrophotometry, and 12 triterpenoids was determined by HPLC with Ultimate XB-C18 column(4.6 mm×150 mm, 5 μm)and the mobile phase consisting of acetonitrile-water(5% methanol)at a flow rate of 1.0 mL · min-1.The detection wavelength was set at 208 nm. With 23-acetate alisol B as the standard, the UV-Vis spectrophotometry method was applied.There was a good linear relationship between the concentration and absorbance of 23-acetate alisol B in the range of 5.9-15.6 μg · mL-1 (r=0.999 1);the average recovery rate was 99.05% and the RSD was 2.3%.By HPLC, the linear range of 12 detected compounds were fine(r>0.999 0), the overall recoveries ranged from 95.89%-99.33%, with the RSD ranging from 2.0%-3.4%.The contents of 12 triterpenoids including alisol A, alisol B, alisol C, alisol G, alisol L, 23-acetate-alisol A, 23-acetate-alisol B, 23-a cetate-alisol C, 16-oxo-23-acetate-alisol A, 16-oxo-24-acetate-alisol A, 11-Deoxyalisol B, 24-a cetate-alisol A were 5.71%-5.95%, 3.47%-3.99%, 2.53%- 2.97%, 3.07%-3.93%, 3.65%-3.99%, 2.39%-3.02%, 10.56%-11.32%, 9.44%-9.86%, 0.63% -3.14%, 1.08%-1.21%, 2.29%-2.85% and 4.04%-5.02%, respectively.
CONCLUSIONS:
The two methods are in accordance with the requirements of the methodology validation, indicating that the ultraviolet-visible(UV-Vis)spectrophotometry method can be used for the detection of the total triterpenoids and the HPLC method can be used for detection of 12 triterpenoids. |
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