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    Natural Products
    6''-O-acetylsaikosaponin A
    6''-O-acetylsaikosaponin A
    Information
    CAS No. 64340-46-1 Price $268 / 10mg
    Catalog No.CFN95086Purity>=98%
    Molecular Weight823Type of CompoundTriterpenoids
    FormulaC44H70O14Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison
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    Size /Price /Stock 10 mM * 1 mL in DMSO / $249.2 / In-stock
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    6''-O-acetylsaikosaponin A

    6''-O-acetylsaikosaponin A
    Product Name 6''-O-acetylsaikosaponin A
    CAS No.: 64340-46-1
    Catalog No.: CFN95086
    Molecular Formula: C44H70O14
    Molecular Weight: 823 g/mol
    Purity: >=98%
    Type of Compound: Triterpenoids
    Physical Desc.: Powder
    Source: The roots of Bupleurum chinense DC.
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Price: $268 / 10mg
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  • Related Screening Libraries
    Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
    10 mM * 1 mL in DMSO / Inquiry / In-stock
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    Description: Reference standards.
    6''-O-acetylsaikosaponin A Description
    Source: The roots of Bupleurum chinense DC.
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.2151 mL 6.0753 mL 12.1507 mL 24.3013 mL 30.3767 mL
    5 mM 0.243 mL 1.2151 mL 2.4301 mL 4.8603 mL 6.0753 mL
    10 mM 0.1215 mL 0.6075 mL 1.2151 mL 2.4301 mL 3.0377 mL
    50 mM 0.0243 mL 0.1215 mL 0.243 mL 0.486 mL 0.6075 mL
    100 mM 0.0122 mL 0.0608 mL 0.1215 mL 0.243 mL 0.3038 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    J Pharm Biomed Anal. 2009 May 1;49(4):1048-55.
    Fast determination of saikosaponins in Bupleurum by rapid resolution liquid chromatography with evaporative light scattering detection.[Pubmed: 19201128 ]
    A rapid resolution liquid chromatography coupled with evaporative light scattering detection method was established for simultaneous determination of six saikosaponins, namely saikosaponin a, saikosaponin c, saikosaponin d, 6''-O-acetylsaikosaponin A, 3''-O-acetylsaikosaponin d and 6''-O-acetylsaikosaponin d in Bupleurum.
    METHODS AND RESULTS:
    The analysis was performed by using an Agilent Zorbax SB-C18 column (1.8 microm, 3.0 mm x 50 mm i.d.) at gradient elution of water and acetonitrile, and the saikosaponins were well separated within 12 min, which provided about a fourfold reduction in analysis time by comparing a conventional high performance liquid chromatography method. Owing to their low ultraviolet absorption, the saikosaponins were detected by evaporative light scattering. The standard curves to quantify the saikosaponins were constructed by the log-log plot, which showed good linearity with the correlation coefficients exceeding 0.9954. The detection limits and quantification limits ranged in 8.38-25.00 microg/mL and 25.13-45.00 microg/mL, respectively. Satisfactory intra-day and inter-day precisions were achieved with the relative standard deviation (R.S.D.) less than 6.58%, and the average recoveries obtained were in the range of 96.9-100.4%. In addition, MeOH-1.0% (v/v) pyridine was found to be the best the extraction solvent when compared to MeOH and MeOH-1.0% (v/v) ammonia water. A total of 23 samples of roots of Bupleurum from different species or locations were examined with this analytical method, and their chemical profiles provided information for the chemotaxonomic investigation.
    CONCLUSIONS:
    The results demonstrated that the analytical method is highly effective for the quality evaluation of Bupleurum species.
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