| Structure Identification: |
| Plant Cell Reports, 1991, 10(2):85-89. | | Agrobacterium rhizogenes-mediated transformation ofEchinacea purpurea.[Reference: WebLink] |
METHODS AND RESULTS:
Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transformation of E. purpurea.
CONCLUSIONS:
Comparative HPLC fingerprint analysis of the alkamides from natural plant source, control tissues, and transformed callus and roots indicated that transformed callus and hairy roots might be a promising source for continuous and standardized production of theDodeca 2E,4E,8Z,10E,Z-tetraenoic acid isobutylamide and related amides. | | American journal of plant sciences, 2015, 6(12):201-212. | | Effect of Extraction Methods on the Active Compounds and Antioxidant Properties of Ethanolic Extracts of Echinacea purpurea Flower.[Reference: WebLink] |
METHODS AND RESULTS:
The extraction yields, active compounds and antioxidant properties of 50%-aqueous-ethanolic extracts of freeze-dried Echinacea purpurea flower with multi-steps and multi-batches extraction methods were assessed. In multi-steps extraction, the extraction yields of 1st, 2nd, and 3rd extracts were 21.52%, 9.33%, and 2.90%, and their total phenols contents were 182.08, 176.33, and 177.08 mg CAE/g, respectively, with cichoric acid (62.07-66.57 mg/g) being the main phenolic compound. No differences in the contents of individual and total caffeic acids derivates existed among 1st, 2nd, and 3rd extracts.
The Dodeca 2E,4E,8Z,10E,Z-tetraenoic acid isobutylamide(alkamide 8/9) contents of 1st, 2nd, and 3rd extracts were 505.38, 598.61, and 585.99 µg/g, respectively. In multi-batches extraction, the extracted dry weight increased with increasing the sample batches, with the extraction yields and alkamide 8/9 contents of samples decreased from 19.93% to 12.98% and 534.36 to 269.76 µg/g, respectively.
CONCLUSIONS:
The total phenol (177.25-186.92 mg CAE/g), individual and total caffeic acid derivatives (85.99-95.06 mg/g) contents of extracts among different sample batches were not significantly different, with cichoric acid (63.66-70.31 mg/g) being the main phenolic compound.
All the prepared extracts also exhibited potent anti-oxidant properties. Overall, the two-step sequential extraction is desirable for extracting bioactive compounds from freeze-dried E. purpurea flower. |
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)