Ecdysone | CAS:3604-87-3 | Manufacturer ChemFaces

Ecdysone

Citing Use of our Products

How to Order

Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Sent to Email: info@chemfaces.com

Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Fax: (0086)-27-84254680
E-mail: manager@chemfaces.com
Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC

Delivery time

Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal & Western Union

* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.

According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.

Size /Price /Stock	10 mM * 1 mL in DMSO / Inquiry
Other Packaging	*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap

Featured Products

Afzelin

Catalog No: CFN98757
CAS No: 482-39-3
Price: $288/20mg

Decuroside I

Catalog No: CFN95004
CAS No: 96638-79-8
Price: $318/5mg

meso-Hannokinol

Catalog No: CFN90864
CAS No: N/A
Price: $368/5mg

Dictamnine

Catalog No: CFN99783
CAS No: 484-29-7
Price: $100/20mg

Salvianolic acid B

Catalog No: CFN99332
CAS No: 115939-25-8
Price: $50/20mg

20-Deoxocarnosol

Catalog No: CFN92175
CAS No: 94529-97-2
Price: $463/5mg

Ginsenoside Re

Catalog No: CFN99974
CAS No: 52286-59-6
Price: $40/20mg

Description:

Ecdysone signaling through Ecdysone receptor isoform B1 is required cell autonomously for the muscle death. A nctional Bombyx Ecdysone receptor binds to EcRE-D and activates the expression of BmBR-C. Mnoaminergic autocrine signaling in the PG regulates Ecdysone biogenesis in a coordinated fashion on activation by PTTH and Ilps.

Targets:

Sodium Channel | ATPase | Potassium Channel | Autophagy

In vitro:

Dev Biol. 2015 Feb 15;398(2):163-76.

Ecdysone regulates morphogenesis and function of Malpighian tubules in Drosophila melanogaster through EcR-B2 isoform.[Pubmed: 25476260]

Malpighian tubules are the osmoregulatory and detoxifying organs of Drosophila and its proper development is critical for the survival of the organism. They are made up of two major cell types, the ectodermal principal cells and mesodermal stellate cells. The principal and stellate cells are structurally and physiologically distinct from each other, but coordinate together for production of isotonic fluid. Proper integration of these cells during the course of development is an important pre-requisite for the proper functioning of the tubules.
METHODS AND RESULTS:
We have conclusively determined an essential role of Ecdysone hormone in the development and function of Malpighian tubules. Disruption of Ecdysone signaling interferes with the organization of principal and stellate cells resulting in malformed tubules and early larval lethality. Abnormalities include reduction in the number of cells and the clustering of cells rather than their arrangement in characteristic wild type pattern. Organization of F-actin and β-tubulin also show aberrant distribution pattern. Malformed tubules show reduced uric acid deposition and altered expression of Na(+)/K(+)-ATPase pump.
CONCLUSIONS:
B2 isoform of Ecdysone receptor is critical for the development of Malpighian tubules and is expressed from early stages of its development.

Insect Mol Biol. 2014 Jun;23(3):341-56.

Ecdysone response elements in the distal promoter of the Bombyx Broad-Complex gene, BmBR-C.[Pubmed: 24576019]

METHODS AND RESULTS:
As determined by a luciferase assay, the transcriptional activity of Pdist, but not Pprox, was activated by Ecdysone. Further analyses using reporters driven by sequential deletion Pdist mutants indicated that two regions, Ecdysone responsive element (EcRE)-D and EcRE-P, -4950 bp and -3480 bp upstream from the distal transcription start site, respectively, were important in the responsiveness of Pdist to 20-hydroxyEcdysone (20E); however, no significant sequence similarities were found between the canonical EcRE and the EcRE-D or EcRE-P regions. Electrophoretic mobility shift assays showed that both the EcRE-D and -P sequences specifically bound to Bombyx protein(s). Sequence analyses and competition assays suggested that the protein(s) bound to EcRE-P might include components other than the Ecdysone receptor (EcR), suggesting that BmBR-C transcription was indirectly activated by Ecdysone through the EcRE-P. Remarkably, protein binding to the mid-region of the EcRE-D, EcRE-Db, was competitively inhibited by an oligonucleotide containing the Drosophila hsp27 EcRE sequence. Furthermore, an anti-EcR antibody interfered with the formation of the protein-EcRE-Db complex.
CONCLUSIONS:
These results indicated that a functional Bombyx Ecdysone receptor binds to EcRE-D and activates the expression of BmBR-C.

Ecdysone Description

Source:	The roots of Cyanotis arachnoidea C. B. Clarke
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Kinase Assay:

Proc Natl Acad Sci U S A. 2015 Feb 3;112(5):1452-7.

Autocrine regulation of ecdysone synthesis by β3-octopamine receptor in the prothoracic gland is essential for Drosophila metamorphosis.[Pubmed: 25605909]

In Drosophila, pulsed production of the steroid hormone Ecdysone plays a pivotal role in developmental transitions such as metamorphosis. Ecdysone production is regulated in the prothoracic gland (PG) by prothoracicotropic hormone (PTTH) and insulin-like peptides (Ilps).
METHODS AND RESULTS:
Here, we show that monoaminergic autocrine regulation of Ecdysone biosynthesis in the PG is essential for metamorphosis. PG-specific knockdown of a monoamine G protein-coupled receptor, β3-octopamine receptor (Octβ3R), resulted in arrested metamorphosis due to lack of Ecdysone. Knockdown of tyramine biosynthesis genes expressed in the PG caused similar defects in Ecdysone production and metamorphosis. Moreover, PTTH and Ilps signaling were impaired by Octβ3R knockdown in the PG, and activation of these signaling pathways rescued the defect in metamorphosis.
CONCLUSIONS:
Thus, monoaminergic autocrine signaling in the PG regulates Ecdysone biogenesis in a coordinated fashion on activation by PTTH and Ilps. We propose that monoaminergic autocrine signaling acts downstream of a body size checkpoint that allows metamorphosis to occur when nutrients are sufficiently abundant.

Dev Biol. 2013 Nov 15;383(2):275-84.

Ecdysone signaling at metamorphosis triggers apoptosis of Drosophila abdominal muscles.[Pubmed: 24051228]

One of the most dramatic examples of programmed cell death occurs during Drosophila metamorphosis, when most of the larval tissues are destroyed in a process termed histolysis. Much of our understanding of this process comes from analyses of salivary gland and midgut cell death. In contrast, relatively little is known about the degradation of the larval musculature.
METHODS AND RESULTS:
Here, we analyze the programmed destruction of the abdominal dorsal exterior oblique muscle (DEOM) which occurs during the first 24h of metamorphosis. We find that Ecdysone signaling through Ecdysone receptor isoform B1 is required cell autonomously for the muscle death. Furthermore, we show that the orphan nuclear receptor FTZ-F1, opposed by another nuclear receptor, HR39, plays a critical role in the timing of DEOM histolysis. Finally, we show that unlike the histolysis of salivary gland and midgut, abdominal muscle death occurs by apoptosis, and does not require autophagy.
CONCLUSIONS:
Thus, there is no set rule as to the role of autophagy and apoptosis during Drosophila histolysis.

Animal Research:

Dev Biol. 2014 Mar 15;387(2):229-39.

INO80-dependent regression of ecdysone-induced transcriptional responses regulates developmental timing in Drosophila.[Pubmed: 24468295]

Sequential pulses of the steroid hormone Ecdysone regulate the major developmental transitions in Drosophila, and the duration of each developmental stage is determined by the length of time between Ecdysone pulses. Ecdysone regulates biological responses by directly initiating target gene transcription. In turn, these transcriptional responses are known to be self-limiting, with mechanisms in place to ensure regression of hormone-dependent transcription. However, the biological significance of these transcriptional repression mechanisms remains unclear.
METHODS AND RESULTS:
Here we show that the chromatin remodeling protein INO80 facilitates transcriptional repression of Ecdysone-regulated genes during prepupal development. In ino80 mutant animals, inefficient repression of transcriptional responses to the late larval Ecdysone pulse delays the onset of the subsequent prepupal Ecdysone pulse, resulting in a significantly longer prepupal stage. Conversely, increased expression of ino80 is sufficient to shorten the prepupal stage by increasing the rate of transcriptional repression. Furthermore, we demonstrate that enhancing the rate of regression of the mid-prepupal competence factor βFTZ-F1 is sufficient to determine the timing of head eversion and thus the duration of prepupal development.
CONCLUSIONS:
Although ino80 is conserved from yeast to humans, this study represents the first characterization of a bona fide ino80 mutation in any metazoan, raising the possibility that the functions of ino80 in transcriptional repression and developmental timing are evolutionarily conserved.

CAS No.	3604-87-3	Price
Catalog No.	CFN90487	Purity	>=98%
Molecular Weight	464.63	Type of Compound	Steroids
Formula	C₂₇H₄₄O₆	Physical Description	Cryst.
Download	COA MSDS SDF	Similar structural	Comparison (Web) (SDF)

Product Name	Ecdysone
CAS No.:	3604-87-3
Catalog No.:	CFN90487
Molecular Formula:	C₂₇H₄₄O₆
Molecular Weight:	464.63 g/mol
Purity:	>=98%
Type of Compound:	Steroids
Physical Desc.:	Cryst.
Targets:	Sodium Channel \| ATPase \| Potassium Channel \| Autophagy
Source:	The roots of Cyanotis arachnoidea C. B. Clarke
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Price:
Download:	COA MSDS SDF
Similar structural:	Comparison (Web) (SDF)

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Moulting hormone activity Compound Library Steroids Compound Library Sodium Channel Inhibitor Library Potassium Channel Inhibitor Library Autophagy Inhibitor Library ATPase Inhibitor Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.1523 mL	10.7613 mL	21.5225 mL	43.045 mL	53.8063 mL
5 mM	0.4305 mL	2.1523 mL	4.3045 mL	8.609 mL	10.7613 mL
10 mM	0.2152 mL	1.0761 mL	2.1523 mL	4.3045 mL	5.3806 mL
50 mM	0.043 mL	0.2152 mL	0.4305 mL	0.8609 mL	1.0761 mL
100 mM	0.0215 mL	0.1076 mL	0.2152 mL	0.4305 mL	0.5381 mL

CFN93571	25S-Inokosterone	19595-18-7	480.6	C₂₇H₄₄O₇
CFN93572	25R-Inokosterone	19682-38-3	480.6	C₂₇H₄₄O₇
CFN90217	Turkesterone	41451-87-0	496.64	C₂₇H₄₄O₈
CFN90487	Ecdysone	3604-87-3	464.63	C₂₇H₄₄O₆
CFN90522	Rhapontisterone B	698975-64-3	480.63	C₂₇H₄₄O₇
CFN98873	20-Hydroxyecdysone	5289-74-7	480.6	C₂₇H₄₄O₇
CFN89545	Polypodine B	18069-14-2	496.63	C₂₇H₄₄O₈
CFN98229	Ecdysterone 20,22-monoacetonide	22798-96-5	520.7	C₃₀H₄₈O₇
CFN98230	Ecdysterone 2,3:20,22-diacetonide	22798-98-7	560.8	C₃₃H₅₂O₇
CFN90027	Vitamin D3	67-97-0	384.64	C₂₇H₄₄O