Science | Nature | Cell | View More
Natural Products
L-Stepholidine
L-Stepholidine
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name L-Stepholidine
Price: $318 / 20mg
CAS No.: 16562-13-3
Catalog No.: CFN90457
Molecular Formula: C19H21NO4
Molecular Weight: 327.37 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Powder
Source: The roots of Stephania japonica.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $270.3 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • Lund University (Sweden)
  • Indian Institute of Science (India)
  • University of Leipzig (Germany)
  • Guangzhou Institutes of Biomedi... (China)
  • Florida A&M University (USA)
  • University of Stirling (United Kingdom)
  • Osmania University (India)
  • Amity University (India)
  • VIT University (India)
  • Seoul National University of Sc... (Korea)
  • Universite de Lille1 (France)
  • More...
Package
Featured Products
Isorhoifolin

Catalog No: CFN98927
CAS No: 552-57-8
Price: $318/10mg
Laricitrin

Catalog No: CFN70400
CAS No: 53472-37-0
Price: $ / mg
Sophoricoside

Catalog No: CFN90148
CAS No: 152-95-4
Price: $40/20mg
Ascorbic acid

Catalog No: CFN90048
CAS No: 50-81-7
Price: $30/20mg
20(R)-Ginsenoside Rg3

Catalog No: CFN98170
CAS No: 38243-03-7
Price: $60/20mg
Peimine

Catalog No: CFN99996
CAS No: 23496-41-5
Price: $50/20mg
Oxypaeoniflorin

Catalog No: CFN99589
CAS No: 39011-91-1
Price: $138/20mg
Isoliquiritin apioside

Catalog No: CFN90800
CAS No: 120926-46-7
Price: $338/20mg
Proline

Catalog No: CFN99612
CAS No: 147-85-3
Price: $30/20mg
2,4-Dihydroxybenzoic acid

Catalog No: CFN70215
CAS No: 89-86-1
Price: $30/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: L-Stepholidine, which has dual actions on dopamine D1 and D2 receptors, attenuates heroin self-administration and cue-induced reinstatement. It also elicits anti-dyskinesia effects.
Targets: Dopamine Receptor | 5-HT Receptor
In vivo:
Neuroreport. 2014 Jan 8;25(1):7-11.
L-Stepholidine, a naturally occurring dopamine D1 receptor agonist and D2 receptor antagonist, attenuates heroin self-administration and cue-induced reinstatement in rats.[Pubmed: 24145772]
Opiate addiction is a chronic, relapsing brain disease characterized by persistent and uncontrolled drug-seeking behavior despite negative effects. L-Stepholidine (L-SPD) is an alkaloid extract of the Chinese herb Stephania intermedia with dopamine D1 receptor partial agonistic and D2 receptor antagonistic dual actions. The unique pharmacological profile of L-SPD suggests that L-SPD may be effective for the treatment of opiate addiction.
METHODS AND RESULTS:
The aim of this study was to characterize the effects of L-SPD on heroin self-administration on a fixed-ratio 1 schedule and cue-induced reinstatement under an extinction/reinstatement protocol. The effect of L-SPD on the locomotor activity of heroin-free rats was also tested. We found that 2.5, 5, and 10 mg/kg of L-SPD attenuated heroin self-administration and cue-induced reinstatement without affecting locomotor activity.
CONCLUSIONS:
These results showed that L-SPD, which has dual actions on dopamine D1 and D2 receptors, attenuates heroin self-administration and cue-induced reinstatement.
Neurobiol Aging. 2010 Jun;31(6):926-36.
L-stepholidine reduced L-DOPA-induced dyskinesia in 6-OHDA-lesioned rat model of Parkinson's disease.[Pubmed: 18707801]
L-3,4-dihydroxyphenylalanine (L-DOPA)-induced dyskinesia (LID) remains a challenge in Parkinson's disease (PD) drug therapy.
METHODS AND RESULTS:
In the present study, we examined the effect of L-Stepholidine (L-SPD), a known dual dopamine receptor agent, on LID in 6-hydroxydopamine (6-OHDA)-lesioned PD rat model. Daily administration of L-DOPA to PD rats for 22 days induced steady expression of LID, co-administration of L-Stepholidine with L-DOPA significantly ameliorated LID without compromising the therapeutic potency of L-DOPA, indicating that L-Stepholidine attenuated LID development. L-Stepholidine alone elicited stable contralateral rotational behavior without inducing significant dyskinesia. Acute administration of L-Stepholidine to rats with established LID produced significant relief of dyskinesia; this effect was mimicked by D(2) receptor antagonist haloperidol, but blunted by 5-HT(1A) receptor antagonist WAY100635. Furthermore, the mRNA level of 5-HT(1A) decreased significantly on 6-OHDA-lesioned striata, whereas chronic L-Stepholidine treatment restored 5-HT(1A) receptor mRNA level on the lesioned striata.
CONCLUSIONS:
The present data demonstrated that L-Stepholidine elicited antidyskinesia effects via both dopamine (D(2) receptor antagonistic activity) and nondopamine (5-HT(1A) agonistic activity) mechanisms.
L-Stepholidine Description
Source: The roots of Stephania japonica.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Diosmin Impurity 5

Catalog No: CFN95311
CAS No: 122087-66-5
Price: $318/10mg
Gymnoside III

Catalog No: CFN95033
CAS No: 899430-03-6
Price: $238/10mg
Oleuroside

Catalog No: CFN95099
CAS No: 116383-31-4
Price: $288/20mg
Tortoside B (Manglieside E)

Catalog No: CFN95390
CAS No: 190655-17-5
Price: $413/5mg
Hainanic acid B

Catalog No: CFN95526
CAS No: 1637737-46-2
Price: $413/5mg
Maingayone

Catalog No: CFN95479
CAS No: 271585-66-1
Price: $318/10mg
(-)-5'-Methoxyisolariciresinol 3al...

Catalog No: CFN95403
CAS No: 143236-03-7
Price: $318/5mg
Ganosinensic acid C

Catalog No: CFN95567
CAS No: 2231756-23-1
Price: $413/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.0546 mL 15.2732 mL 30.5465 mL 61.093 mL 76.3662 mL
5 mM 0.6109 mL 3.0546 mL 6.1093 mL 12.2186 mL 15.2732 mL
10 mM 0.3055 mL 1.5273 mL 3.0546 mL 6.1093 mL 7.6366 mL
50 mM 0.0611 mL 0.3055 mL 0.6109 mL 1.2219 mL 1.5273 mL
100 mM 0.0305 mL 0.1527 mL 0.3055 mL 0.6109 mL 0.7637 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Synapse. 2011 May;65(5):379-87.
l-Stepholidine-induced excitation of dopamine neurons in rat ventral tegmental area is associated with its 5-HT(1A) receptor partial agonistic activity.[Pubmed: 20803620]
We studied the effects of L-Stepholidine on the activity of dopamine (DA) neurons in the ventral tegmental area (VTA) using in vivo single-unit recording technique in rats.
METHODS AND RESULTS:
We found that L-Stepholidine increased VTA DA neurons firing rate and induced slow oscillation in firing pattern. Moreover, L-Stepholidine, not clozapine, reversed d-amphetamine-induced inhibition which induced an excitation of VTA DA neurons. Furthermore, our data indicated that the excitatory effect of L-Stepholidine is associated with its partial agonistic action for the 5-HT(1A) receptor since the 5-HT(1A) receptor antagonist WAY100635 could block the L-Stepholidine-induced excitatory effect. However, activation of 5-HT(1A) receptor alone by specific agonist (±)-8-Hydroxy-2-(dipropylamino) tetralin (8-OH-DPAT) was insufficient to elicit excitation of VTA DA neurons, but the excitation of 8-OH-DPAT on VTA DA neurons was elicited in the presence of D₂-like receptors antagonist raclopride. Collectively, these results indicate that l-SPD excited VTA DA neurons requiring its D₂-like receptors antagonistic activity and 5-HT(1A) receptor agonistic activity.
CONCLUSIONS:
The present data demonstrate that D₂ receptor antagonist/5-HT(1A) receptor agonistic dual properties modulate dopaminergic transmission in a unique pattern that may underlie the different therapeutic responses between L-Stepholidine and other atypical antipsychotic drugs.
Animal Research:
Neurosci Lett. 2014 Jan 24;559:67-71.
L-stepholidine, a natural dopamine receptor D1 agonist and D2 antagonist, inhibits heroin-induced reinstatement.[Pubmed: 24269875]
L-Stepholidine (l-SPD), an alkaloid extract of the Chinese herb Stephania intermedia, is the first compound known to exhibit mixed dopamine D1 receptor agonist/D2 antagonist properties and is a potential medication for the treatment of opiate addiction. The aim of the present study was to investigate the effects of pretreatment with L-SPD on heroin-seeking behavior induced by heroin priming.
METHODS AND RESULTS:
Male Sprague-Dawley rats were trained to self-administer heroin (0.05mg/kg per infusion) under a fixed ratio 1 schedule for 12 consecutive days and nose-poke responding was extinguished for 12 days, after which reinstatement of drug seeking was induced by heroin priming. Pretreatment with L-SPD (2.5, 5.0 and 10.0mg/kg, i.p.) inhibited the heroin-induced reinstatement of heroin-seeking behavior. Importantly, L-SPD did not affect locomotion, indicating that the observed effects of L-SPD on reinstatement are not the result of motor impairments.
CONCLUSIONS:
The present data suggested that l-SPD inhibits heroin-induced reinstatement and its potential for the treatment of heroin relapse.
Isorhoifolin

Catalog No: CFN98927
CAS No: 552-57-8
Price: $318/10mg
Laricitrin

Catalog No: CFN70400
CAS No: 53472-37-0
Price: $ / mg
Sophoricoside

Catalog No: CFN90148
CAS No: 152-95-4
Price: $40/20mg
Ascorbic acid

Catalog No: CFN90048
CAS No: 50-81-7
Price: $30/20mg
20(R)-Ginsenoside Rg3

Catalog No: CFN98170
CAS No: 38243-03-7
Price: $60/20mg
Peimine

Catalog No: CFN99996
CAS No: 23496-41-5
Price: $50/20mg
Oxypaeoniflorin

Catalog No: CFN99589
CAS No: 39011-91-1
Price: $138/20mg
Isoliquiritin apioside

Catalog No: CFN90800
CAS No: 120926-46-7
Price: $338/20mg
Proline

Catalog No: CFN99612
CAS No: 147-85-3
Price: $30/20mg
2,4-Dihydroxybenzoic acid

Catalog No: CFN70215
CAS No: 89-86-1
Price: $30/20mg
Tags: buy L-Stepholidine | L-Stepholidine supplier | purchase L-Stepholidine | L-Stepholidine cost | L-Stepholidine manufacturer | order L-Stepholidine | L-Stepholidine distributor