Manassantin B | CAS:88497-88-5 | Manufacturer ChemFaces

Manassantin B

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Description:

Manassantin B is a potent inhibitor of NF-κB activation by the suppression of transciptional activity of RelA/p65 subunit of NF-κB. It also possesses anti-EBV lytic replication activity. Manassantin B inhibits interleukin-6-induced signal transducer and activator of transcription 3 activation in Hep3B cells, it has potential as a potent anti-inflammatory drug for use in pathological processes such as sepsis or acute lung injury. Manassantin B exerts antifibrotic activity in HSC-T6 cells, in part, via inhibition of cell proliferation and decrease of collagen production.

Targets:

NF-κB | p65 | EBV | TNF-α | Bcl-2 | p38 MAPK | JNK | ERK | IL Recepter | STAT

In vitro:

Journal of Natural Products,2013, 77(1):100-110.

Potential antiviral lignans from the roots of Saururus chinensis with activity against Epstein-Barr virus lytic replication.[Reference: WebLink]

Epstein-Barr virus (EBV) is a member of the γ-herpes virus subfamily and has been implicated in the pathogenesis of several human malignancies.
METHODS AND RESULTS:
Bioassay-guided fractionation was conducted on an EtOAc-soluble extract of the roots of Saururus chinensis and monitored using an EBV lytic replication assay. This led to the isolation of 19 new (1-19) and nine known (20-28) lignans. The absolute configurations of the new lignans were established by Mosher's ester, ECD, and computational methods. Eight lignans, including three sesquineolignans (19, 23, and 24) and five dineolignans (3, 4, 26, 27, and 28), exhibited inhibitory effects toward EBV lytic replication with EC50 values from 1.09 to 7.55 μM and SI values from 3.3 to 116.4. In particular, Manassantin B (27) exhibited the most promising inhibition, with an EC50 of 1.72 μM, low cytotoxicity, CC50 > 200 μM, and SI > 116.4.
CONCLUSIONS:
This is the first study demonstrating that lignans possess anti-EBV lytic replication activity.

Natural Product Sciences, 2008, 14(2):118-121.

Antifibrotic activity of manassantin B from Saururus chinensis in HSC-T6 hepatic stellate cells.[Reference: WebLink]

METHODS AND RESULTS:
Manassantin B, a dilignan isolated from Saururus chinensis, significantly inhibited proliferation in HSC-T6 cells in concentration- and time-dependent manners. In addition, treatment of HSC-T6 cells with Manassantin B changed cell morphology from flattened myofibroblastic membranous morphology, representing activation state, to slender shape, representing quiescent state. Furthermore, Manassantin B effectively reduced collagen content in HSC-T6 cells.
CONCLUSIONS:
These results suggested that Manassantin B exerted antifibrotic activity in HSC-T6 cells, in part, via inhibition of cell proliferation and decrease of collagen production.

Manassantin B Description

Source:	The herbs of Saururus chinensis
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Kinase Assay:

Biochemical Pharmacology, 2003, 66(10):1925-1933.

Suppression of RelA/p65 transactivation activity by a lignoid manassantin isolated from Saururus chinensis.[Reference: WebLink]

In our search for NF-κB inhibitors from natural resources, we have previously identified two structurally related dilignans, manassantin A and Manassantin B as specific inhibitors of NF-κB activation from Saururus chinensis. However, their molecular mechanism of action remains unclear.
METHODS AND RESULTS:
We here demonstrate that manassantin A and Manassantin B are potent inhibitors of NF-κB activation by the suppression of transciptional activity of RelA/p65 subunit of NF-κB. These compounds significantly inhibited the induced expression of NF-κB reporter gene by LPS or TNF-α in a dose-dependent manner. However, these compounds did not prevent the DNA-binding activity of NF-κB assessed by electrophoretic mobility shift assay as well as the induced-degradation of IκB-α protein by LPS or TNF-α. Further analysis revealed that manassantin A and Manassantin B dose-dependently suppressed not only the induced NF-κB activation by overexpression of RelA/p65, but also transactivation activity of RelA/p65. Furthermore, treatment of cells with these compounds prevented the TNF-α-induced expression of anti-apoptotic NF-κB target genes Bfl-1/A1, a prosurvival Bcl-2 homologue, and resulted in sensitizing HT-1080 cells to TNF-α-induced cell death. Similarly, these compounds also suppressed the LPS-induced inducible nitric oxide synthase expression and nitric oxide production.
CONCLUSIONS:
Taken together, manassantin A and Manassantin B could be valuable candidate for the intervention of NF-κB-dependent pathological condition such as inflammation and cancer.

Cell Research:

Korean Journal of Anesthesiology, 2012, 62(2):161-5.

Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells.[Reference: WebLink]

Elevated systemic levels of pro-inflammatory cytokines cause hypotension during septic shock and induce capillary leakage in acute lung injury. Manassantin B has anti-inflammatory and anti-plasmoidal properties. This study examined the effects of Manassantin B on lipopolysaccharide (LPS)-induced inflammatory response in murine macrophages.
METHODS AND RESULTS:
RAW 264.7 macrophage cells were incubated without or with (1, 3 and 10 µM) Manassantin B and without or with (100 ng/ml) LPS. Manassantin B dissolved in phosphate buffered saline was added to the medium 1 h prior to the addition of LPS. The degree of activation of mitogen-activated protein kinase (MAPK) including extracellular signal-regulated kinases 1 and 2 (ERK1/2), c-Jun amino terminal kinases (JNK) and p38 MAPK, and the level of interleukin (IL)-1β were determined 30 min and 24 h after the addition of LPS respectively. Manassantin B inhibited the production of IL-1β and attenuated the phosphorylations of ERK1/2 and p38 MAPK, but not that of JNK, in RAW 264.7 cells treated with LPS. Manassantin B reduces LPS-induced IL-1β expression through effects on ERK1/2- and p38 MAPK-mediated pathways.
CONCLUSIONS:
Manassantin B has potential as a potent anti-inflammatory drug for use in pathological processes such as sepsis or acute lung injury.

Product Name	Manassantin B
Price:	$388 / 20mg
CAS No.:	88497-88-5
Catalog No.:	CFN70466
Molecular Formula:	C₄₁H₄₈O₁₁
Molecular Weight:	716.8 g/mol
Purity:	>=98%
Type of Compound:	Lignans
Physical Desc.:	Powder
Source:	The herbs of Saururus chinensis
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS
Similar structural:	Comparison (Web) (SDF)
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Lignans Compound Library TNF-α Inhibitor Library STAT Inhibitor Library p65 Inhibitor Library JNK Inhibitor Library ERK Inhibitor Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	1.3951 mL	6.9754 mL	13.9509 mL	27.9018 mL	34.8772 mL
5 mM	0.279 mL	1.3951 mL	2.7902 mL	5.5804 mL	6.9754 mL
10 mM	0.1395 mL	0.6975 mL	1.3951 mL	2.7902 mL	3.4877 mL
50 mM	0.0279 mL	0.1395 mL	0.279 mL	0.558 mL	0.6975 mL
100 mM	0.014 mL	0.0698 mL	0.1395 mL	0.279 mL	0.3488 mL

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CFN95443	Fragransin B1	112516-03-7	404.5	C₂₂H₂₈O₇
CFN00451	Veraguensin	19950-55-1	372.5	C₂₂H₂₈O₅
CFN90914	Chicanine	78919-28-5	342.39	C₂₀H₂₂O₅
CFN96728	Futokadsurin C	852459-91-7	356.41	C₂₁H₂₄O₅
CFN90655	rel-(8R,8'R)-dimethyl-(7S,7'R)-bis(3,4-methylenedioxyphenyl)tetrahydro-furan	178740-32-4	340.4	C₂₀H₂₀O₅
CFN95244	Henricine	107783-46-0	386.4	C₂₂H₂₆O₆
CFN92890	(-)-di-de-O-methylgrandisin	50393-98-1	404.45	C₂₂H₂₈O₇
CFN95361	Foliachinenoside C	1041180-87-3	598.6	C₂₈H₃₈O₁₄
CFN95267	Saucerneol	88497-86-3	538.6	C₃₁H₃₈O₈