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    Natural Products
    ent-Kauran-17,19-dioic acid
    Information
    CAS No. 60761-79-7 Price
    Catalog No.CFN97033Purity>=98%
    Molecular Weight334.5 Type of CompoundDiterpenoids
    FormulaC20H30O4Physical DescriptionPowder
    Download     COA    MSDS    SDFSimilar structuralComparison (Web)  (SDF)
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    According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
    Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
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    ent-Kauran-17,19-dioic acid

    ent-Kauran-17,19-dioic acid
    Product Name ent-Kauran-17,19-dioic acid
    CAS No.: 60761-79-7
    Catalog No.: CFN97033
    Molecular Formula: C20H30O4
    Molecular Weight: 334.5 g/mol
    Purity: >=98%
    Type of Compound: Diterpenoids
    Physical Desc.: Powder
    Source: The herbs of Siegesbeckia orientalis
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Price:
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    Description: Reference standards.
    ent-Kauran-17,19-dioic acid Description
    Source: The herbs of Siegesbeckia orientalis
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 2.9895 mL 14.9477 mL 29.8954 mL 59.7907 mL 74.7384 mL
    5 mM 0.5979 mL 2.9895 mL 5.9791 mL 11.9581 mL 14.9477 mL
    10 mM 0.299 mL 1.4948 mL 2.9895 mL 5.9791 mL 7.4738 mL
    50 mM 0.0598 mL 0.299 mL 0.5979 mL 1.1958 mL 1.4948 mL
    100 mM 0.0299 mL 0.1495 mL 0.299 mL 0.5979 mL 0.7474 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    Journal of Separation Science, 2012, 35(19).
    Simultaneous determination of seven major diterpenoids in Siegesbeckia pubescensMakino by high‐performance liquid chromatography coupled with evaporative light scattering detection[Reference: WebLink]

    METHODS AND RESULTS:
    A novel HPLC method with evaporative light scattering detection was developed for the simultaneous quantification of seven major diterpenoids of two types, including ent‐pimarane type: Kirenol, Hythiemoside B, Darutigenol, and ent‐kaurane type: ent‐16β,17,18‐trihydroxy‐kauran‐19‐oic acid, ent‐17,18‐dihydroxy‐kauran‐19‐oic acid, ent‐16β,17‐dihydroxy‐kauran‐19‐oic acid, 16α‐hydro‐ent-Kauran-17,19-dioic acid in the aerial parts of Siegesbeckia pubescens Makino, an important traditional Chinese medicinal herb. Chromatographic separation was achieved on a Waters Symmetry ShieldTM RP18 column (250 mm× 4.6 mm id, 5 μm) with a gradient mobile phase (A: 0.3% v/v aqueous formic acid and B: acetonitrile) at a flow rate of 1.0 mL/min. The drift tube temperature of evaporative light scattering detection was set at 103°C, and nitrogen flow rate was 3.0 L/min. The method was validated for accuracy, precision, LOD, and LOQ. All calibration curves showed a good linear relationship (r > 0.999) in test range. Precision was evaluated by intra‐ and interday tests that showed RSDs were less than 3.5%. Accuracy validation showed that the recovery was between 96.5 and 102.0% with RSDs below 2.8%.
    CONCLUSIONS:
    The validated method was successfully applied to determine the contents of seven diterpenoids in the different parts of Siegesbeckia pubescens Makino from two sources and to determine the contents of ent‐pimarane, ent‐kaurane, and total diterpenoids.
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