| Description: |
(+)-Bicuculline is a competitive antagonist of GABAA receptors with IC50 of 2 μM, also blocks Ca(2+)-activated potassium channels.It is effective in vitro against spore germination of some plant pathogenic fungi, it can significantly inhibit spore germination of all the fungi at concentrations of 100-1000 ppm. |
| Targets: |
GABA Receptor | Calcium Channel | Antifection |
| In vitro: |
| Folia Microbiol (Praha). 2002;47(2):161-5. | | Effect of berberine and (+/-)-bicuculline isolated from Corydalis chaerophylla on spore germination of some fungi.[Pubmed: 12058395] |
METHODS AND RESULTS:
Berberine and (+)-Bicuculline were isolated from roots and leaves, respectively, of Corydalis chaerophylla. Both were effective in vitro against spore germination of some plant pathogenic fungi (Alternaria brassicicola, A. brassicae, A. cheiranthi, A. melongenae, A. solani, Colletotrichum musae, C. falcatum, Curvularia penniseti, C. lunata, C. maculans, C. pallescens, Curvularia sp., Erysiphe pisi, E. cichoracearum, Erysiphe sp., Fusarium udum, Helminthosporium spiciferum, H. penniseti, H. frumentacei, Heterosporium sp., Oidium erysiphoides and Ustilago cynodontis). Berberine and (+)-Bicuculline significantly inhibited spore germination of all the fungi at concentrations of 100-1000 ppm.
CONCLUSIONS:
Berberine was effective against all the fungi at all concentrations; most of the fungi did not germinate at 1000 ppm. H. penniseti conidia did not germinate at any concentration of (+)-Bicuculline . U. cynodontis was the least sensitive fungus at lower concentrations but 800 ppm dose was highly effective. |
|
| In vivo: |
| Exp. Brain Res., 1999, 129(3):451-6. | | The effects of adrenalectomy and gonadectomy on sex and stress-induced differences in the sensitivity of rats to bicuculline[Reference: WebLink] |
METHODS AND RESULTS:
The effects of adrenalectomy, gonadectomy and combined adrenalectomy plus gonadectomy on previously described sex-dependent anticonvulsive effect of swim stress was studied in rats. The convulsive signs (myoclonic twitch, generalized convulsions, tonic hindlimb extension) were produced by constant i.v. infusion of GABAA antagonist bicuculline, which started 15 min after termination of swim stress (10-min swim at 18-19 0 C). In the same animals swim stress postponed, especially in females, the onset of the first myoclonic twitch and generalized convulsions, but not the onset of tonic hindlimb extension.
CONCLUSIONS:
In summary, our results suggest that hormones of the adrenal and gonadal glands are only partly responsible for decreased susceptibility, especially of female rats, to GABAA antagonist bicuculline. Moreover, they have demonstrated that stress produces gender-specific anticonvulsive effect even in the animals completely deprived of steroid hormones of the peripheral origin. |
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)