• ChemFaces is a professional high-purity natural products manufacturer.
  • Product Intended Use
  • 1. Reference standards
  • 2. Pharmacological research
  • 3. Inhibitors
  • Home
  • Natural Products
  • Bioactive
  • Screening Libraries
  • Hot Products
  • Plant Catalog
  • Customer Support
  • Product Use Citation
  • About Us
  • Contact Us
  • Natural Products
    Isotetrandrine
    Information
    CAS No. 477-57-6 Price
    Catalog No.CFN98722Purity>=98%
    Molecular Weight622.8 Type of CompoundAlkaloids
    FormulaC38H42N2O6Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
    Citing Use of our Products
    How to Order
    Orders via your E-mail:

    1. Product number / Name / CAS No.
    2. Delivery address
    3. Ordering/billing address
    4. Contact information
    Sent to Email: info@chemfaces.com
    Contact Us
    Order & Inquiry & Tech Support

    Tel: (0086)-27-84237683
    Fax: (0086)-27-84254680
    E-mail: manager@chemfaces.com
    Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
    Delivery time
    Delivery & Payment method

    1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

    2. We accept: Wire transfer & Credit card & Paypal & Western Union
    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
    Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
    Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
    Our products had been exported to the following research institutions and universities, And still growing.
  • University of Oslo (Norway)
  • Max-Planck-Insitut (Germany)
  • VIT University (India)
  • Universitas islam negeri Jakarta (Indonesia)
  • The Australian National University (Australia)
  • Griffith University (Australia)
  • Massachusetts General Hospital (USA)
  • Pennsylvania State University (USA)
  • Medizinische Universit?t Wien (Austria)
  • Medical University of Gdansk (Poland)
  • Instituto de Investigaciones Ag... (Chile)
  • More...
  • Package
    Featured Products
    Voacangine

    Catalog No: CFN92252
    CAS No: 510-22-5
    Price: $463/5mg
    Narcissoside

    Catalog No: CFN99773
    CAS No: 604-80-8
    Price: $188/20mg
    Quercetin-3-gentiobioside

    Catalog No: CFN93585
    CAS No: 7431-83-6
    Price: $298/10mg
    Syringin

    Catalog No: CFN99282
    CAS No: 118-34-3
    Price: $80/20mg
    Picroside III

    Catalog No: CFN99567
    CAS No: 64461-95-6
    Price: $228/20mg
    Neochamaejasmine A

    Catalog No: CFN92151
    CAS No: 90411-13-5
    Price: $418/5mg
    Dihydrokavain

    Catalog No: CFN90536
    CAS No: 587-63-3
    Price: $318/20mg
    Related Screening Libraries
    Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
    10 mM * 1 mL in DMSO / Inquiry / In-stock
    Related Libraries
  • Anti-inflammatory Compound Library
  • Antioxidants Compound Library
  • Inhibitors Compound Library
  • Antibacterial Compound Library
  • Antiviral Compound Library
  • Alkaloids Compound Library
  • Nrf2 Inhibitor Library
  • NF-kB Inhibitor Library
  • MAPK Inhibitor Library
  • JNK Inhibitor Library
  • IL Receptor Inhibitor Library
  • HO-1 Inhibitor Library
  • Biological Activity
    Description: Isotetrandrine is a small molecule inhibitor, on various aspects of LPS-induced inflammation in vitro and in vivo. It dose-dependently suppresses the severity of LPS-induced ALI by inactivation of MAPK and NF-κB, which may involve the inhibition of tissue oxidative injury and pulmonary inflammatory process.
    Targets: IL Receptor | NF-kB | MAPK | Nrf2 | HO-1 | JNK
    In vitro:
    J Surg Res. 2014 Apr;187(2):596-604.
    Isotetrandrine protects against lipopolysaccharide-induced acute lung injury by suppression of mitogen-activated protein kinase and nuclear factor-kappa B.[Pubmed: 24331940]
    Mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-κB) signaling pathways are pleiotropic regulator of many genes involved in lipopolysaccharide (LPS)-induced acute lung injury (ALI). The present study aimed to reveal the protective effect of Isotetrandrine (ITD), a small molecule inhibitor, on various aspects of LPS-induced inflammation in vitro and in vivo.
    METHODS AND RESULTS:
    In vitro, RAW 264.7 cells were pretreated with different dose of Isotetrandrine 1 h before treatment with 1 mg/L of LPS. In vivo, to induce ALI, male BALB/c mice were injected intranasally with LPS and treated with Isotetrandrine (20 and 40 mg/kg) 1 h before LPS. In vitro, the cytokine levels of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in supernatant were reduced by Isotetrandrine. Meanwhile, in vivo, pulmonary inflammatory cell infiltration, myeloperoxidase activity, total cells, neutrophils, macrophages, along with the levels of tumor necrosis factor-α, IL-1β, and IL-6 in bronchoalveolar lavage fluid were dose-dependently attenuated by Isotetrandrine. Furthermore, our data showed that Isotetrandrine significantly inhibited the activation of MAPK and NF-κB, which are induced by LPS in ALI model.
    CONCLUSIONS:
    These results suggested that Isotetrandrine dose-dependently suppressed the severity of LPS-induced ALI by inactivation of MAPK and NF-κB, which may involve the inhibition of tissue oxidative injury and pulmonary inflammatory process.
    Isotetrandrine Description
    Source: The roots of Stephania tetrandra S.Moore
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Recent ChemFaces New Products and Compounds
    1-Methoxyphaseollidin

    Catalog No: CFN96878
    CAS No: 65428-13-9
    Price: $413/5mg
    Lappaol A

    Catalog No: CFN95067
    CAS No: 62333-08-8
    Price: $333/10mg
    Methyl 5-O-feruloylquinate

    Catalog No: CFN92445
    CAS No: 154461-64-0
    Price: $516/5mg
    Isololiolide

    Catalog No: CFN95106
    CAS No: 38274-00-9
    Price: $388/5mg
    Curzerenone

    Catalog No: CFN92027
    CAS No: 20493-56-5
    Price: $258/10mg
    Sagittatoside A

    Catalog No: CFN90139
    CAS No: 118525-35-2
    Price: $350/5mg
    Dihydrowithaferin A

    Catalog No: CFN91055
    CAS No: 5589-41-3
    Price: $258/5mg
    Naringin 4'-glucoside

    Catalog No: CFN95014
    CAS No: 17257-21-5
    Price: $338/5mg
    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.
    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.6057 mL 8.0283 mL 16.0565 mL 32.113 mL 40.1413 mL
    5 mM 0.3211 mL 1.6057 mL 3.2113 mL 6.4226 mL 8.0283 mL
    10 mM 0.1606 mL 0.8028 mL 1.6057 mL 3.2113 mL 4.0141 mL
    50 mM 0.0321 mL 0.1606 mL 0.3211 mL 0.6423 mL 0.8028 mL
    100 mM 0.0161 mL 0.0803 mL 0.1606 mL 0.3211 mL 0.4014 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Kinase Assay:
    Exp Biol Med (Maywood). 2016 Aug;241(14):1568-76.
    Isotetrandrine ameliorates tert-butyl hydroperoxide-induced oxidative stress through upregulation of heme oxygenase-1 expression.[Pubmed: 27190261]
    1R, 1'S-Isotetrandrine, a naturally occurring plant alkaloid found in Mahonia of Berberidaceae, possesses anti-inflammatory, antibacterial, and antiviral properties, but the antioxidative activity and mechanism action remain unclear.
    METHODS AND RESULTS:
    In this study, we demonstrated the antioxidative effect and mechanism of 1R, 1'S-Isotetrandrine against tert-butyl hydroperoxide-induced oxidative damage in HepG2 cells. We found that 1R, 1'S-Isotetrandrine suppressed cytotoxicity, reactive oxygen species generation, and glutathione depletion. Additionally, our study confirmed that 1R, 1'S-Isotetrandrine significantly increased the antioxidant enzyme heme oxygenase-1 expression and nuclear translocation of factor-erythroid 2 p45-related factor 2 (Nrf2). Specifically, the nuclear translocation of Nrf2 induced by 1R, 1'S-Isotetrandrine was associated with Nrf2 negative regulatory protein Keap1 inactivation and phosphorylation of both extracellular signal-regulated protein kinase and c-Jun NH2-terminal kinase. Preincubation with thiol-reducing agents reduced 1R, 1'S-Isotetrandrine-induced heme oxygenase-1 expression, and treatment with either extracellular signal-regulated protein kinase or c-Jun NH2-terminal kinase inhibitors attenuated the levels of 1R, 1'S-Isotetrandrine-induced Nrf2 activation and heme oxygenase-1 expression. Furthermore, the cytoprotective effect of 1R, 1'S-Isotetrandrine was abolished by heme oxygenase-1, extracellular signal-regulated protein kinase, and c-Jun NH2-terminal kinase inhibitors.
    CONCLUSIONS:
    These results indicated that the 1R, 1'S-Isotetrandrine ameliorated tert-butyl hydroperoxide-induced oxidative damage through upregulation of heme oxygenase-1 expression by the dissociation of Nrf2 from Nrf2-Keap1 complex via extracellular signal-regulated protein kinase and c-Jun NH2-terminal kinase activation and Keap1 inactivation.