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More articles cited ChemFaces products.
Environ Toxicol.2020, doi: 10.1002Antiviral Res.2013, 98(3):386-93Natural Product Communications...2020...Heliyon.2023, e12684.Ulm University Medical Center...2020...Phytomedicine.2022, 100:154058.Front Pharmacol.2021, 12:744624.Int J Mol Sci.2022, 23(1):538.
J Pain Res.2022, 15:3469-3478.Anal Bioanal Chem. 2016, 408(15)Molecules.2022, 27(22):7887.Sci Rep. 2018, 10590Life Sci.2018, 209:498-506FEBS Lett.2015, 589(1):182-7Plants (Basel).2021, 10(4):702.Int J Mol Sci.2020, 21(19):7209.
J Ethnopharmacol.2022, 289:115018. Molecules.2022, 27(21):7514.Oncology Letters2018, 4690-4696Phytomedicine.2018, 40:37-47Evid Based Complement Alternat Me...2015...Phytochemistry Letters2021, 43:80-87.Nutrients.2018, 10(12):E1998
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Biochem Biophys Res Commun.2018, 495(1):1271-1277Phytother Res.2019, 33(3):676-689National University of Pharmacy2022, 1:73-76Mol Immunol. 2016, 78:121-132Bulletin of Health Research2016, 44(4):279-286Sci Rep. 2017, 12953(7)Front Pharmacol.2021, 12:615157.Free Radic Biol Med.2021, 166:104-115.Preprints2017, 2017120176J Sep Sci.2020, 43(22):4148-4161.
Related Screening Libraries
||Usnic acid has antitumoral, acaricidal, larvicidal, antiviral, antibiotic, antipyretic, analgesic,gastroprotective, antioxidative and anti-inflammatory activities. Use of reconstituted bovine type-I collagen-based films containing usnic acid can improve burn healing process in rats. Usnic acid perturbs various interrelated signaling pathways and that autophagy induction is a defensive mechanism against usnic acid-induced cytotoxicity; it disturbs calcium homeostasis, induces ER stress, and that Usnic acid-induced cellular damage occurs at least partially via activation of the Ca(2+) channel of SOCE.
||Calcium Channel | Autophagy | Caspase | JNK | SOD | TNF-α | IL Receptor | NOS | Antifection|
|Parasitol Res. 2014 Jun;113(6):2387-90. |
|Acaricidal activity of usnic acid and sodium usnic acid against Psoroptes cuniculi in vitro.[Pubmed: 24770718]|
|Usnic acid, a major active compound in lichens, was first isolated in 1884. Since then, Usnic acid and its sodium salt (sodium Usnic acid) have been used in medicine, perfumery, cosmetics, and other industries due to its extensive biological activities. However, its acaricidal activity has not been studied. In this paper, we investigated the acaricidal activity of Usnic acid and sodium Usnic acid against Psoroptes cuniculi in vitro.
METHODS AND RESULTS:
After evaluating the acaricidal activity and toxicity of Usnic acid and sodium Usnic acid in vitro, the results showed that at doses of 250, 125, and 62.5 mg/ml, Usnic acid and sodium Usnic acid can kill mites with 91.67, 85.00, and 55.00% and 100, 100, and 60.00% mortality after treatment 24 h. The LT50 values were 4.208, 8.249, and 16.950 h and 3.712, 7.339, and 15.773 h for Usnic acid and sodium Usnic acid, respectively.
Sodium Usnic acid has a higher acaricidal activity than Usnic acid, which may be related to the difference in their structures.
|Z Naturforsch C. 2006 Nov-Dec;61(11-12):773-6. |
|Identification and quantitation of usnic acid from the lichen Usnea species of Anatolia and antimicrobial activity.[Pubmed: 17294685]|
|Six species of lichens, such as Usnea florida, Usnea barbata, Usnea longissima, Usnea rigida, Usnea hirta and Usnea subflorida, were collected from different areas of Anatolia (district of Antalya, Karabük, Qankiri, Giresun and Trabzon) in Turkey.
METHODS AND RESULTS:
Their Usnic acid amounts in acetone extracts were determined by HPLC. In addition, antimicrobial activities of these extracts were determined against Escherichia coli (ATCC 35218), Enterococcus faecalis (RSKK 508), Proteus mirabilis (Pasteur Ens. 235), Staphylococcus aureus, Bacillus subtilis and Bacillus megaterium.
It was shown that with increasing amount of Usnic acid, the antimicrobial activity increased. Usnic acid contents of Usnea species varied between 0.22-6.49% of dry weight.
|Lat. Am. J. Pharm., 2009, 300(2): R349-60. |
|Larvicidal Activity of Cladonia substellata Extract and Usnic Acid against Aedes aegypti and Artemia salina[Reference: WebLink]|
|The chloroform extract of Cladonia substellata Vainio was assayed against larvae of Aedes ae- gypti, the mosquito vector of Dengue fever and Artemia salina.
METHODS AND RESULTS:
The extract was tested at concentrations ranging from 1 to 15 ppm in an aqueous medium for 24 h. LC50 and LC90 were evaluated. Since the chlo- roform extract proved to be lethal for third to fourth instar larvae, downstream processing was undertak- en to purify the active agents in the extract. The major compound in the chloroform extract was purified by crystallization followed by column chromatography to yield yellow crystals. Furthermore, Usnic acid (UA) was evaluated for its larvicidal potential. The major compound in the chloroform extract, UA, exhib- ited LC50 of 6.6 ppm (6.1 to 7.0 ppm). Therefore, UA is most likely the active principle in C. substellata.
UA showed to be toxic to A. salina, a reference organism in assays to evaluate the potential toxicity hazard to invertebrates in ecosystems.
|Int Immunopharmacol. 2014 Oct;22(2):371-8. |
|Usnic acid protects LPS-induced acute lung injury in mice through attenuating inflammatory responses and oxidative stress.[Pubmed: 25068825]|
|Usnic acid is a dibenzofuran derivative found in several lichen species, which has been shown to possess several activities, including antiviral, antibiotic, antitumoral, antipyretic, analgesic, antioxidative and anti-inflammatory activities. However, there were few reports on the effects of Usnic acid on LPS-induced acute lung injury (ALI). The aim of our study was to explore the effect and possible mechanism of Usnic acid on LPS-induced lung injury.
METHODS AND RESULTS:
In the present study, we found that pretreatment with Usnic acid significantly improved survival rate, pulmonary edema. In the meantime, protein content and the number of inflammatory cells in bronchoalveolar lavage fluid (BALF) significantly decreased, and the levels of MPO, MDA, and H2O2 in lung tissue were markedly suppressed after treatment with Usnic acid. Meanwhile, the activities of SOD and GSH in lung tissue significantly increased after treatment with Usnic acid. Additionally, to evaluate the anti-inflammatory activity of Usnic acid, the expression of pro-inflammatory cytokines including tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and anti-inflammatory cytokine IL-10, and chemokines interleukin-8 (IL-8) and macrophage inflammatory protein-2 (MIP-2) in BALF were studied. The results in the present study indicated that Usnic acid attenuated the expression of TNF-α, IL-6, IL-8 and MIP-2. Meanwhile, the improved level of IL-10 in BALF was observed.
In conclusion, these data showed that the protective effect of Usnic acid on LPS-induced ALI in mice might relate to the suppression of excessive inflammatory responses and oxidative stress in lung tissue. Thus, it was suggested that Usnic acid might be a potential therapeutic agent for ALI.
|J Biomed Biotechnol. 2011;2011:761593. |
|Collagen-based films containing liposome-loaded usnic acid as dressing for dermal burn healing.[Pubmed: 21274404]|
|The aim of this study was assess the effect of collagen-based films containing Usnic acid as a wound dressing for dermal burn healing.
METHODS AND RESULTS:
Second-degree burn wounds were performed in forty-five Wistar rats, assigned into nine groups: COL-animals treated with collagen-based films; PHO-animals treated with collagen films containing empty liposomes; UAL-animals treated with collagen-based films containing Usnic acid incorporated into liposomes. After 7, 14, and 21 days the animals were euthanized. On 7th day there was a moderate infiltration of neutrophils, in UAL, distributed throughout the burn wounds, whereas in COL and PHO, the severity of the reaction was slighter and still limited to the margins of the burn wounds. On the 14th day, the inflammatory reaction was less intense in UAL, with remarkable plasma cells infiltration. On the 21st day, there was reduction of the inflammation, which was predominantly composed of plasma cells in all groups, particularly in UAL. The use of the Usnic acid provided more rapid substitution of type-III for type-I collagen on the 14th day, and improved the collagenization density on the 21st day.
It was concluded that the use of reconstituted bovine type-I collagen-based films containing Usnic acid improved burn healing process in rats.
Usnic acid Description
||From usnea diffracta Vain.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)PMID: 29328914
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)PMID: 32004475
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)PMID: 29149595
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)PMID: 29553709
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)PMID: 28005066
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Toxicol Sci. 2015 Jul;146(1):116-26. |
|Endoplasmic Reticulum Stress and Store-Operated Calcium Entry Contribute to Usnic Acid-Induced Toxicity in Hepatic Cells.[Pubmed: 25870318 ]|
|The use of Usnic acid as a weight loss agent is a safety concern due to reports of acute liver failure in humans. Previously we demonstrated that Usnic acid induces apoptosis and cytotoxicity in hepatic HepG2 cells. We also demonstrated that Usnic acid induces autophagy as a survival mechanism against its cytotoxicity. In this study, we investigated and characterized further molecular mechanisms underlying the toxicity of Usnic acid in HepG2 cells.
METHODS AND RESULTS:
We found that Usnic acid causes endoplasmic reticulum (ER) stress demonstrated by the increased expression of typical ER stress markers, including CHOP, ATF-4, p-eIF2α, and spliced XBP1. Usnic acid inhibited the secretion of Gaussia luciferase measured by an ER stress reporter assay. An ER stress inhibitor 4-phenylbutyrate attenuated Usnic acid-induced apoptosis. Moreover, Usnic acid significantly increased the cytosolic free Ca(2+) concentration. Usnic acid increased the expression of calcium release-activated calcium channel protein 1 (CRAM1 or ORAI1) and stromal interaction molecule 1, two key components of store-operated calcium entry (SOCE), which is the major Ca(2+) influx pathway in non-excitable cells, this finding was also confirmed in primary rat hepatocytes. Furthermore, knockdown of ORAI1 prevented ER stress and ATP depletion in response to Usnic acid. In contrast, overexpression of ORAI1 increased ER stress and ATP depletion caused by Usnic acid.
Taken together, our results suggest that Usnic acid disturbs calcium homeostasis, induces ER stress, and that Usnic acid-induced cellular damage occurs at least partially via activation of the Ca(2+) channel of SOCE.
|Toxicol Sci. 2014 Nov;142(1):33-44. |
|The role of autophagy in usnic acid-induced toxicity in hepatic cells.[Pubmed: 25078063]|
|The use of Usnic acid and Usnic acid-containing products is associated with acute liver failure; however, mechanistic studies of hepatotoxicity caused by Usnic acid are limited. In this study, we investigated and characterized the possible mechanisms, especially the role of autophagy in Usnic acid's toxicity in human HepG2 cells.
METHODS AND RESULTS:
Usnic acid caused apoptosis as demonstrated by an increased caspase-3/7 activity and an increased subdiploid nucleus formation. Usnic acid-induced autophagy as demonstrated by the conversion of LC3B-I to LC3B-II, degradation of P62, and an increased number of puncta. Inhibition of autophagy by treating cells with autophagy inhibitors (3-methyladenine or chloroquine) or by small interfering RNA against Atg7 aggravated Usnic acid-induced apoptosis and decreased cell viability, indicating that autophagy plays a protective role against Usnic acid-induced toxicity. Moreover, Usnic acid activated the MAPK signaling pathway. Usnic acid-elicited apoptosis was enhanced and autophagy was decreased when JNK was suppressed by a specific inhibitor. Additionally, inhibition of autophagy decreased the activity of JNK.
Taken together, our results suggest that Usnic acid perturbs various interrelated signaling pathways and that autophagy induction is a defensive mechanism against Usnic acid-induced cytotoxicity.
|J Ethnopharmacol. 2006 Jan 3;103(1):59-65. |
|Gastroprotective and antioxidant effects of usnic acid on indomethacin-induced gastric ulcer in rats.[Pubmed: 16169175 ]|
|Usnea longissima, a medicinal lichen of Anatolia (Turkey), is used in the treatment of gastric ulcer in local folk medicine.
METHODS AND RESULTS:
In this paper, the gastroprotective effect of Usnic acid (UA) isolated from Usnea longissima was investigated in the indomethacin-induced gastric ulcers in rats at doses of 25, 50, 100 and 200 mg/kg body weight. The gastric lesions were significantly reduced by all doses of UA as compared with the indomethacin (25 mg/kg body weight) treated group. In the stomach tissues of treated animals, the in vivo antioxidant levels were evaluated. The administration of indomethacin caused a significant decrease in the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH), and an increase in the lipid peroxidation (LPO) level (p < 0.05). The administration of all doses of UA reversed the trend, inducing a significant increase of SOD, GSH and GPx levels and a reduction in LPO level in tissues. However, catalase (CAT), glutathione reductase (GR) and myeloperoxidase (MPx) activities, increased by indomethacin, were found to be lower in the UA- and ranitidine-treated groups. The gastric mucosal constitutive NO synthase (cNOS) and inducible NO synthase (iNOS) activities were also investigated in tissues of UA- (100 mg/kg), ranitidine- (50 mg/kg) and indomethacin-treated rat groups. The administration of UA and ranitidine increased the cNOS activity and lowered the iNOS activity as compared with indomethacin-treated group.
These results suggest that the gastroprotective effect of UA can be attributed to its reducing effect on the oxidative damage and neutrophil infiltration in tissues.
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