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    Rotundine
    Information
    CAS No. 10097-84-4 Price $30 / 20mg
    Catalog No.CFN99195Purity>=98%
    Molecular Weight355.42Type of CompoundAlkaloids
    FormulaC21H25NO4Physical DescriptionWhite powder
    Download Manual    COA    MSDSSimilar structuralComparison (Web)
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    Biological Activity
    Description: Rotundine provides remarkable protection against cerebral ischemia reperfusion damage, its mechanisms may be through its influence on NO、ET 1 and energy metabolism. Rotundine injection can ameliorate the damages by modulating the activities of different types of NOS. It combined with methadone treatment may be a better therapeutic method in treatment of heroin dependence.
    Targets: NOS | NO
    In vivo:
    Chinese Journal of Drug Abuse Prevention & Treatment, 2006, 12(5):270-1.
    Therapeutic Effects of Rotundine Combined with Methadone in Treatment of Heroin Dependence[Reference: WebLink]
    To explore the therapeutic effects of Rotundine combined with methadone in treatment of heroin dependence.
    METHODS AND RESULTS:
    Methods: Sixty heroin addicts were randomly divided into two groups. Group A were treated with Rotundine plus methadone and group B were treated with methadone alone. Results: The effective rate of group A was significantly higher than that of group B (96.67% and 73.33%, respectively, P 0.05). The total dose of methadone and the days of treatment in group A (275±21.2 mg and 8±2.3 d, respectively) was significantly lower than that of group B (415±31.2 mg and 12±2.5 d, respectively, P 0.01 and P 0.05, respectively).
    CONCLUSIONS:
    Conclusion: Rotundine combined with methadone treatment may be a better therapeutic method in treatment of heroin dependence.
    Journal of Emergenoy Medicine, 2000, 9(6):379-81.
    Influence of rotundine on NO、ET-1 and energy metabolism after acute brain injury resulted from complete cerebral ischemia reperfusion in the rats[Reference: WebLink]

    METHODS AND RESULTS:
    To find the influence of Rotundine(L THP) on NO、ET 1、ATP and Lactate in rat brain tissues after the global ischemia reperfusion and to investigate protective effects of L THP.Methods:The four vessel occlusion model was made as described by Pulsinelli Brierlog in Sprange Daweley rats.The influences of L THP on NO、ET 1 and energy metabolism during the reperfusion period was observed.Results:L THP can suppress the marked increase of NO、ET 1 and lactate in the early stage of reperfusion,and also improve the contents of ATP.
    CONCLUSIONS:
    L THP provides remarkable protection against cerebral ischemia reperfusion damage.Its mechanisms may be through its influence on NO、ET 1 and energy metabolism.
    Rotundine Description
    Source: The root of Stephania tetrandra S. Moore
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 2.8136 mL 14.0679 mL 28.1357 mL 56.2715 mL 70.3393 mL
    5 mM 0.5627 mL 2.8136 mL 5.6271 mL 11.2543 mL 14.0679 mL
    10 mM 0.2814 mL 1.4068 mL 2.8136 mL 5.6271 mL 7.0339 mL
    50 mM 0.0563 mL 0.2814 mL 0.5627 mL 1.1254 mL 1.4068 mL
    100 mM 0.0281 mL 0.1407 mL 0.2814 mL 0.5627 mL 0.7034 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Animal Research:
    Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 Jun;19(6):361-4.
    Effects of rotundine injection on nitric oxide synthase in tissues of different organs after ischemia/reperfusion of brain in rats.[Pubmed: 17577446]
    To explore the protective effect of Rotundine injection on lung, liver and kidney damages after cerebral ischemia/reperfusion (I/R) injury in rats based on the activity changes of nitric oxide synthase (NOS).
    METHODS AND RESULTS:
    Seventy-six rats were randomly divided into three groups: sham operation group, I/R injury group and treatment group, and determinations were done at five different time points. The cerebral I/R models were reproduced by improved 4 vessels occlusion method. The activities of NOS in the lung, liver and kidney were measured in all the rats at 2, 6, 12, 24 and 48 hours after reperfusion. Compared with sham operation group, the activities of total NOS (tNOS) were significantly increased at 2, 12 and 24 hours in I/R injury group (P<0.05 or P<0.01), with the peak value at 12 hours (all P<0.01). The activities of constitutive NOS (cNOS) were increased significantly at 2 hours (all P<0.05), and those of induced NOS (iNOS) were increased at 12 hours (all P<0.01). The activities of iNOS were still high at 24 hours (all P<0.05), and approached the levels of sham operation group at 48 hours. Compared with I/R injury group, the activities of cNOS in various organs increased much higher at 2 hours in treatment group (all P<0.05). But those of iNOS were significantly decreased after 12 hours (P<0.05 or P<0.01).
    CONCLUSIONS:
    The various types of NOS play different roles in the lung damages after brain I/R injury at different stages in rats. Rotundine injection can ameliorate the damages by modulating the activities of different types of NOS.
    Structure Identification:
    Guang Pu Xue Yu Guang Pu Fen Xi. 2014 Nov;34(11):2989-93.
    IR and Raman spectra studies of Rotundine based on DFT.[Pubmed: 25752044]
    Infrared spectroscopy (IR), the normal Raman spectroscopy (NRS) and the surface enhanced Raman spectroscopy (SERS) in new Ag/Cu nanomaterial of Rotundine were studied in the present paper.
    METHODS AND RESULTS:
    The IR and the NRS of Rotundine were calculated by the density functional theory (DFT) using B3LYP/6-311+G(d, p), then the spectral intensity graph of Rotundine were given. The vibrational peaks were assigned comprehensively by the visualization software of Gauss view 5. 0. Rotundine has obvious infrared and Raman vibrational peak in the wave number range of 3 300-2500 and 1 800-600 cm(-1). SnCl2 and PVP was used as capping agent for the silver nanoparticles in SERS of Rotundine. Finally, by using the method of cyclic immersion well dispersed silver nanoparticles was obtained and achieved good enhancement effect. This molecule acquired strong selective enhancement vibration peak, In the wave number ranges of 1 500-1 400 and 1 000-700 cm(-1) the enhancement effect is most obvious. After analyzed, the methylene of this molecule is adsorbed on the silver nanoparticles surface and the angle between the benzene ring and the silver substrate is close to 90 degrees. The theoretically calculated spectra of Rotundine are consistent with the obtained experimental spectra. There are some differences may be due to the interaction forces between molecules and so on. The visualization software displayed the structure characteristics and molecular group vibration of this molecular visually and provided important basis for assigning the vibrational peaks.
    CONCLUSIONS:
    Rotundine is an important traditional Chinese medicine agent contained in many kinds of sedative drugs. The study provides a strong basis for the rapid, feature and trace identification of Rotundine and also supplies important reference for the biological role of central inhibition of analgesic drugs.