Info: Read More
  • ChemFaces is a professional high-purity natural products manufacturer.
  • Product Intended Use
  • 1. Reference standards
  • 2. Pharmacological research
  • 3. Inhibitors
  • Home
  • Natural Products
  • Bioactive
  • Screening Libraries
  • Hot Products
  • Plant Catalog
  • Customer Support
  • Product Use Citation
  • About Us
  • Contact Us
  • Science | Nature | Cell | View More
    Natural Products
    CAS No. 29080-58-8 Price $318 / 20mg
    Catalog No.CFN98361Purity>=98%
    Molecular Weight328.3 Type of CompoundFlavonoids
    FormulaC18H16O6Physical DescriptionYellow powder
    Download Manual    COA    MSDS    SDFSimilar structuralComparison (Web)  (SDF)
    Citing Use of our Products
    How to Order
    Orders via your E-mail:

    1. Product number / Name / CAS No.
    2. Delivery address
    3. Ordering/billing address
    4. Contact information
    Sent to Email:
    Contact Us
    Order & Inquiry & Tech Support

    Tel: (0086)-27-84237683
    Fax: (0086)-27-84254680
    Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
    Delivery time
    Delivery & Payment method

    1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

    2. We accept: Wire transfer & Credit card & Paypal & Western Union
    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
    Size /Price /Stock 10 mM * 1 mL in DMSO / $103.4 / In-stock
    Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
    Our products had been exported to the following research institutions and universities, And still growing.
  • Nanjing University of Chinese M... (China)
  • Universiti Kebangsaan Malaysia (Malaysia)
  • Universite Libre de Bruxelles (Belgium)
  • Florida A&M University (USA)
  • Universidad de Ciencias y Artes... (Mexico)
  • University of the Basque Country (Spain)
  • Cancer Research Initatives Foun... (Malaysia)
  • CSIRO - Agriculture Flagship (Australia)
  • University of Maryland School o... (USA)
  • Imperial College London (United Kingdom)
  • Monash University (Australia)
  • More...
  • Package
    Featured Products

    Catalog No: CFN95001
    CAS No: 1891-25-4
    Price: $358/5mg
    Pinoresinol diglucoside

    Catalog No: CFN99994
    CAS No: 63902-38-5
    Price: $100/20mg

    Catalog No: CFN99762
    CAS No: 957-66-4
    Price: $238/10mg

    Catalog No: CFN92425
    CAS No: 86361-55-9
    Price: $218/5mg

    Catalog No: CFN90308
    CAS No: 54984-93-9
    Price: $418/20mg

    Catalog No: CFN92656
    CAS No: 500-64-1
    Price: $158/20mg
    3-O-Caffeoylquinic acid methyl ester

    Catalog No: CFN92573
    CAS No: 123483-19-2
    Price: $198/10mg
    Related Screening Libraries
    Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
    10 mM * 1 mL in DMSO / Inquiry / In-stock
    Related Libraries
  • Anti-inflammatory Compound Library
  • Inhibitors Compound Library
  • Antibacterial Compound Library
  • Antifungal Compound Library
  • Anti-trypanosoma Compound Library
  • Flavonoids Compound Library
  • TNF-α Inhibitor Library
  • PGE Inhibitor Library
  • NOS Inhibitor Library
  • LOX Inhibitor Library
  • IL Receptor Inhibitor Library
  • COX Inhibitor Library
  • Antifection Inhibitor Library
  • Biological Activity
    Description: 5-Hydroxy-3',4',7-trimethoxyflavone has anti-inflammatory, antibacterial and antifungal activities, it also shows moderate antitrypanosomal activity with MIC value of 19.0 ug/ml. 5-Hydroxy-3',4',7-trimethoxyflavone shows prominent inhibitory activity against soybean lipoxygenase, it can significantly inhibit nitric oxide production and induce reduction in the mRNA expressions of inducible nitric oxide synthase and cyclooxygenase-2 in lipopolysaccharide-induced inflammation in RAW 264.7 macrophages.
    Targets: LOX | TNF-α | IL Receptor | PGE | COX | NOS | Antifection
    In vitro:
    Med. Chem. Res.,2016,25(9):1754-67.
    Protective effect of 5-hydroxy-3′,4′,7-trimethoxyflavone against inflammation induced by lipopolysaccharide in RAW 264.7 macrophage: in vitro study and in silico validation.[Reference: WebLink]
    The herb Lippia nodiflora L. (Verbenaceae) has been documented to exhibit anti-inflammatory, antipyretic, antitussive, antidiabetic, anticancer, and antimelanogenesis properties. In the present study, we aimed at evaluating the anti-inflammatory activity of 5-Hydroxy-3',4',7-trimethoxyflavone, a flavonoid from L. nodiflora, using lipopolysaccharide induced inflammation in RAW 264.7 macrophages.
    5-hydroxy-3′,4′,7-trimethoxyflavone significantly inhibited nitric oxide production and demonstrated slight reduction in prostaglandin-E2 level at tested concentrations. The production of pro-inflammatory cytokines, such as TNF-α, IL-6, and IL-1β, were obviously reduced by 5-Hydroxy-3',4',7-trimethoxyflavone in a concentration-dependent manner. Moreover, 5-Hydroxy-3',4',7-trimethoxyflavone significantly induced reduction in the mRNA expressions of inducible nitric oxide synthase and cyclooxygenase-2, representing that inhibition occurs at the transcriptional level. In addition, we performed molecular docking and molecular dynamic simulations to study the interaction of 5-Hydroxy-3',4',7-trimethoxyflavone with inflammatory mediators such as inducible nitric oxide synthase and cyclooxygenase-2. Docking study showed its hydrogen bond interactions with key residues in the active site of inducible nitric oxide synthase and cyclooxygenase-2, enlightening its possible binding mode at the molecular level. The results of molecular dynamic simulations showed the stability of complexes and their interactions.
    Taken together, these findings envisage 5-Hydroxy-3',4',7-trimethoxyflavone as a potential candidate molecule for the progress of therapeutic strategy against inflammation-related diseases.
    Pharm. Biol.,2008, 41(7):483-6.
    Antifungal Flavonoids from Ballota glandulosissima.[Reference: WebLink]
    The flavonoids kumatakenin (1), pachypodol (2), 5-Hydroxy-3',4',7-trimethoxyflavone (3), velutin (4), salvigenin (5), retusin (6) and corymbosin (7) have been isolated from the aerial parts of Ballota glandulosissima Hub.-Mor & Patzak. Among them, 2–4 and 7 have not been reported previously in the genus Ballota.
    The antibacterial and antifungal activities of 1–4 and 6 were tested against Bacillus subtilis, Staphylococcus aureus, Staphylococcus faecalis, Echerichia coli, Pseudomonas aeruginosa, Candida albicans, Candida krusei and Candida galabrata.
    J.Appl. Pharm. Sci.,2016,6(1):102-6.
    Anti-infective Activities of Secondary Metabolites from Vitex pinnata.[Reference: WebLink]
    The phytochemical investigation of Vitex pinnata led to the isolation of a mixture of steroids β-sitosterol and stigmasterol (1a and 1b) and three known flavonoid identified as 5-Hydroxy-3',4',7-trimethoxyflavone(2), 5-hydroxy-7,4’-dimethoxy-flavone (3) and 5-hydroxy-3,3’,4’,7-tetramethoxyflavone (4). The structures of all isolated compounds were carried out by NMR and mass spectrometry.
    The isolated compounds were evaluated for their anti-infective activities against Trypanosoma brucei brucei and Microbacterium marinum. Compound 1-4 showed moderate antitrypanosomal activity with MIC values of 6.25μg/ml, 19.0, 21.0 and 17.0μM, respectively while no activity observed on anti-mycobacterial.
    This study is the first to report the presence of three flavones and their antitrypanosomal activity from V. pinnata.
    5-Hydroxy-3',4',7-trimethoxyflavone Description
    Source: The herbs of Callicarpa bodinieri Levl.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to:

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Recent ChemFaces New Products and Compounds

    Catalog No: CFN93257
    CAS No: 2608-21-1
    Price: $368/5mg
    Dioscoreside E

    Catalog No: CFN95346
    CAS No: 435321-73-6
    Price: $318/5mg

    Catalog No: CFN95198
    CAS No: 75412-95-2
    Price: $318/10mg

    Catalog No: CFN95035
    CAS No: 1422390-59-7
    Price: $368/10mg

    Catalog No: CFN97086
    CAS No: 62949-93-3
    Price: $368/5mg

    Catalog No: CFN90839
    CAS No: 52250-35-8
    Price: $218/10mg

    Catalog No: CFN95186
    CAS No: 131623-13-7
    Price: $318/10mg

    Catalog No: CFN92288
    CAS No: 24404-50-0
    Price: $338/5mg
    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.

    PMID: 30417089
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 3.046 mL 15.23 mL 30.4599 mL 60.9199 mL 76.1499 mL
    5 mM 0.6092 mL 3.046 mL 6.092 mL 12.184 mL 15.23 mL
    10 mM 0.3046 mL 1.523 mL 3.046 mL 6.092 mL 7.615 mL
    50 mM 0.0609 mL 0.3046 mL 0.6092 mL 1.2184 mL 1.523 mL
    100 mM 0.0305 mL 0.1523 mL 0.3046 mL 0.6092 mL 0.7615 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Kinase Assay:
    J Recept Signal Transduct Res. 2015 Jul 9:1-9.
    In vitro, fluorescence-quenching and computational studies on the interaction between lipoxygenase and 5-hydroxy-3',4',7-trimethoxyflavone from Lippia nodiflora L.[Pubmed: 26155726]
    The folk use of L. nodiflora was validated using the isolated natural compound, 5-Hydroxy-3',4',7-trimethoxyflavone (HTMF) by in vitro, fluorescence spectroscopic and molecular modeling studies with lipoxygenase (LOX), because LOX plays an essential role in inflammatory responses.
    In this perspective, the methanol extract and 5-Hydroxy-3',4',7-trimethoxyflavone are shown to demonstrate prominent inhibitory activity against soybean lipoxygenase, with an IC50 value of 21.12 and 23.97 µg/ml, respectively. The data obtained from the spectroscopic method revealed that the quenching of intrinsic fluorescence of LOX is produced as a result of the complex formation of LOX-5-Hydroxy-3',4',7-trimethoxyflavone. The binding mode analysis of 5-Hydroxy-3',4',7-trimethoxyflavone within the LOX enzyme suggested that hydrogen bond formation, hydrophobic interaction and π-π stacking could account for the binding of 5-Hydroxy-3',4',7-trimethoxyflavone. Molecular dynamics results indicated the interaction of 5-Hydroxy-3',4',7-trimethoxyflavone with LOX and the stability of ligand-enzyme complex was maintained throughout the simulation.
    The computational results are reliable with experimental facts and provided a good representation for understanding the binding mode of 5-Hydroxy-3',4',7-trimethoxyflavone inside the active site of lipoxygenase enzyme.
    Journal of Functional Foods, 2015,13:100-7.
    Anti-allergenic activity of polymethoxyflavones from Kaempferia parviflora.[Reference: WebLink]
    The inhibitory effects of 13 polymethoxyflavones (PMFs) obtained from Kaempferia parviflora extracts were evaluated on RBL-2H3 cell degranulation.
    These flavones suppressed antigen-induced degranulation in RBL-2H3 cells. 5-Hydroxy-3',4',7-trimethoxyflavone (KP02) and 5,3′-dihydroxy-3,7,4′,-trimethoxyflavone (KP10) showed potent inhibitory activities. KP10 significantly suppressed calcium ionophores, such as A23187 and 2,5-ditert-butylhydroquinone, which promoted calcium influx from the outside of cells and from the endoplasmic reticulum, respectively. However, these activities did not fully explain the PMFs' effects. Immunoblot analysis of KP02 and KP10 showed that the inhibitory effects were attributable to the downregulation of PLCγ1 kinase phosphorylation in the signaling pathways involved in degranulation. The phosphorylation levels of Syk were also suppressed by the addition of KP10. KP02 and KP10 treatment to the cells did not affect the high affinity IgE receptor (FcεRI) levels inside RBL-2H3 cells, but they suppressed the FcεRI levels in the plasma membrane.
    Therefore, K. parviflora PMFs have multiple degranulation mechanisms to suppress RBL-2H3 cells and would be beneficial for alleviating type I allergy symptoms.