ChemFaces is a professional high-purity natural products manufacturer.
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
|Size /Price /Stock
||10 mM * 1 mL in DMSO / Inquiry||Other Packaging
||*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
More articles cited ChemFaces products.
The Journal of Internal Korean Me...2015...Asian J Beauty Cosmetol...2016...Sci Rep.2016, 6:25094Molecular & Cellular Toxicology...2017...Semyung University2017, 149407Viruses.2017, 9(10)Nutrients.2018, 10(12)PLoS One.2018, 13(3):e0193386
Phys Chem Chem Phys....2018...J Sep Sci.2018, 41(9):1938-1946J Nat Sc Biol Med2019, 10(2):149-156Front Neurosci.2019, 13:1091BMC Complement Altern Med....2019...Food and Fermentation Industries...2019...Phytomedicine.2019, 55:229-237Phytomedicine.2019, 65:153089
Neurochem Int.2020, 133:104629Front Pharmacol.2020, 11:566490.Nutrients.2020, 12(3):595.Int J Mol Sci.2020, 21(22):8816. J Ethnopharmacol.2020, 254:112733. Food Addit Contam Part A Chem Ana...2020...Biochem Biophys Res Commun....2021...
Our products had been exported to the following research institutions and universities, And still growing.
Deutsches Krebsforschungszentrum (Germany)Warszawski Uniwersytet Medyczny (Poland)Institute of Pathophysiology Me... (Austria)Universidade Federal de Goias (... (Brazil)
Universitas islam negeri Jakarta (Indonesia)Korea Food Research Institute(K... (Korea)Regional Crop Research Institute (Korea)Instytut Nawozów Sztucznych w ... (Poland)
VIB Department of Plant Systems... (Belgium)University of Stirling (United Kingdom)Copenhagen University (Denmark)
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1 Building, No. 83, CheCheng Rd., Wuhan Economic and Technological Development Zone, Wuhan, Hubei 430056, PRC
J Ethnopharmacol.2017, 198:91-97BMC Complement Altern Med.2019, 19(1):325Food Sci Nutr.2019, 8(1):246-256Acta Agriculturae Scandinavica2015, 381-383Chemistry of Natural Compounds2018, 204–206Sci Rep. 2018, 462(8)Front Pharmacol.2018, 9:236J Chromatogr B Analyt Technol Biomed Life Sci.2020, 1149:122123.Metabolites.2020, 10(11):440.Front Pharmacol.2018, 9:756
Related Screening Libraries
||Kuguacin J is a promising candidate new antineoplastic and chemopreventive agent for androgen-dependent prostate cancer and carcinogenesis, it exerts growth inhibition through G1 arrest and induction of apoptosis in androgen-dependent human prostate cancer. Kuguacin J modulates the function of P-glycoprotein (P-gp) by directly interacting at the drug-substrate-binding site, and it appears to be an effective inhibitor of P-gp activity in vitro and thus could be developed as an effective chemosensitizer to treat multidrug-resistant cancers.|
||p21 | CDK | Bcl-2/Bax | Caspase | PARP | Androgen Receptor | p53 | P-gp|
|Cancer Lett., 2011, 306(2):142-50. |
|Induction of G1 arrest and apoptosis in androgen-dependent human prostate cancer by Kuguacin J, a triterpenoid from Momordica charantia leaf.[Pubmed: 21429659 ]|
|In this study, we focused on the effects of a bitter melon (Momordica charantia) leaf extract (BMLE) and a purified component, Kuguacin J (KuJ), on androgen-dependent LNCaP human prostate cancer cells.
METHODS AND RESULTS:
Both treatments exerted growth inhibition through G1 arrest and induction of apoptosis. In addition, KuJ markedly decreased the levels of cyclins (D1 and E), cyclin-dependent kinases (Cdk2 and Cdk4) and proliferating cell nuclear antigen, and caused an increase in p21 and p27 levels. Its induction of apoptosis was accompanied by an increase in cleavage of caspase-3 and poly (ADP-ribose) polymerase, attributable to augment of Bax/Bcl-2 and Bad/Bcl-xL and reduction of survivin levels. BMLE and KuJ also reduced the expression of androgen receptor (AR), prostate-specific antigen (PSA) while induced P53 protein level. Down-regulation of p53 by RNA interference indicated that BMLE and KuJ inhibited cell growth partly through p53-dependent cell cycle arrest and apoptotic pathways. Both BMLE and KuJ caused less toxicity in a normal prostate cell line, PNT1A.
Our results suggest that BMLE and a purified component, KuJ, from its diethyl ether fraction could be promising candidate new antineoplastic and chemopreventive agents for androgen-dependent prostate cancer and carcinogenesis.
Kuguacin J Description
||The fruits of Momordica charantia
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)PMID: 29328914
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)PMID: 32004475
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)PMID: 29149595
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)PMID: 29553709
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)PMID: 28005066
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|J. Nutr. Biochem., 2012, 23(1):76-84. |
|Kuguacin J isolated from Momordica charantia leaves inhibits P-glycoprotein (ABCB1)-mediated multidrug resistance.[Pubmed: 21414769]|
|Multidrug resistance (MDR) is a major factor in the failure of chemotherapy in cancer patients. Resistance to chemotherapy has been correlated to the overexpression of ABC drug transporters including P-glycoprotein (P-gp) that actively efflux chemotherapeutic drugs from cancer cells. Our previous study showed that bitter melon (Momordica charantia) leaf extract (BMLE) was able to reverse the MDR phenotype by increasing the intracellular accumulation of chemotherapeutic drugs. |
METHODS AND RESULTS:
In the present study, bioguided fractionation was used to identify the active component(s) of BMLE that is able to modulate the function of P-gp and the MDR phenotype in a human cervical carcinoma cell line (KB-V1). We found that Kuguacin J, one of the active components in BMLE, increased sensitivity to vinblastine and paclitaxel in KB-V1 cells. A flow cytometry assay indicated that Kuguacin J inhibits the transport function of P-gp and thereby significantly increases the accumulation of rhodamine 123 and calcein AM in the cells. These results were confirmed by [3H]-vinblastine transport assay. Kuguacin J significantly increases intracellular [3H]-vinblastine accumulation and decreased the [3H]-vinblastine efflux in the cells. Kuguacin J also inhibited the incorporation of [12⁵I]-iodoarylazidoprazosin into P-gp in a concentration-dependent manner, indicating that Kuguacin J directly interacts with the drug-substrate-binding site on P-gp.
These results indicate that Kuguacin J modulates the function of P-gp by directly interacting at the drug-substrate-binding site, and it appears to be an effective inhibitor of P-gp activity in vitro and thus could be developed as an effective chemosensitizer to treat multidrug-resistant cancers.